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目的 旨在利用网络药理学和生物信息学方法，阐明人参精准抗T细胞衰老的潜在作用机制。方法 通过将T细胞与D氨基半乳糖胺共培养48 h诱导T细胞衰老，并对衰老前后的T细胞进行转录组测序。利用R语言中DEseq2包，识别衰老T细胞的差异表达基因。利用中药系统药理学数据库与分析平台(TCMSP)、中医药整合药理学研究平台(TCMIP)、草药成分和靶标数据库(HIT2)、Pub Chem及Swiss Target Prediction数据库获取人参的活性分子及其药物靶点。利用R语言中ClusterProfiler包进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析进一步阐明靶基因的潜在功能，利用STRING数据库和Cytoscape软件构建靶基因的蛋白-蛋白互作(PPI)网络，并筛选核心基因。最后，通过分子对接技术验证人参活性分子对上述药物靶点的作用效能。结果 共确定了人参抗T细胞衰老的75个靶基因，进一步筛选得到8个核心基因。KEGG通路富集分析发现人参抗T细胞衰老机制主要参与p53信号通路、脂质和动脉粥样硬化、细胞衰老等通路。此外，分子对接结果表明过氧化物酶体增殖物激活受体γ(PPARG)、血红素加氧酶(HMOX)1和B细胞淋巴瘤(Bcl)-2样蛋白(BCL2L)1基因编码蛋白与人参活性分子之间具有稳定的结合能力。结论 网络药理学证明人参通过多靶点、多通路发挥精准抗T细胞衰老作用，并在T细胞衰老相关疾病治疗中具有重大的临床意义和应用前景。
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基本信息:
中图分类号:R285
引用信息:
[1]耿杰,侯传东,陈浩然,等.基于T细胞衰老RNA-seq探究人参精准抗免疫衰老的作用[J].中国老年学杂志,2026,46(06):1032-1038.
基金信息:
国家重点研发计划子课题(2021YFC2701704-1); 国家老年疾病临床医学研究中心“多中心RCT”研究课题(NCRCG-PLAGH-20230010); 军队后勤科研项目保健专项课题(23BJZ25)