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目的 探讨越婢汤加味基于p38丝裂原活化蛋白激酶(MAPK)/核因子红系2相关因子(Nrf)2/活性氧(ROS)对单侧输尿管结扎术(UUO)大鼠氧化应激及肾纤维化的干预机制。方法 44只SD大鼠分为对照组、模型组、马来酸依那普利组、越婢汤加味组，灌胃给药干预14 d后，通过苏木素-伊红(HE)、Masson染色检测肾脏病理变化；实时荧光定量聚合酶链式反应检测肾脏组织中α-平滑肌肌动蛋白(SMA)、E-钙黏蛋白(cadherin)、p38MAPK、Nrf2 mRNA表达量；Western印迹检测肾脏组织中α-SMA、E-cadherin、p-p38MAPK、Nrf2蛋白表达量；比色法检测肾脏组织中ROS、超氧化物歧化酶(SOD)及丙二醛(MDA)含量。结果 与对照组相比，模型组肾小球结构边界相对模糊，肾纤维化明显增多，肾组织中ROS、MDA水平、α-SMA mRNA及蛋白、p-p38MAPK蛋白表达明显上调，SOD水平、E-cadherin、Nrf2、p38MAPK mRNA表达明显下调(P<0.001);与模型组相比，依那普利组和越婢汤加味组肾小球结构边界相对清晰，肾纤维化明显缓解；依那普利组肾组织中MDA、ROS、α-SMA和p-p38MAPK蛋白明显下调，SOD水平、E-cadherin、Nrf2、p38MAPK mRNA及E-cadherin、Nrf2蛋白明显上调(P<0.05,P<0.01,P<0.001);越婢汤加味组肾组织中MDA、ROS水平及p-p38MAPK蛋白表达显著下降，E-cadherin、Nrf2 mRNA及蛋白表达明显升高(P<0.05,P<0.01)。结论 越婢汤加味可以改善UUO所致肾病大鼠肾损伤，其机制可能与调控p38MAPK/Nrf2/ROS信号通路，阻止氧化应激反应，抑制肾纤维化有关。
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基本信息:
中图分类号:R285.5
引用信息:
[1]吕艳杭,姚木铭,杨达龙,等.越婢汤加味通过介导p38MAPK/Nrf2/ROS通路抑制氧化应激减轻UUO大鼠肾脏纤维化[J].中国老年学杂志,2026,46(10):1820-1825.
基金信息:
福建省医学创新课题(2021CXA047); 福建省中医药科技项目(2025YBB020); 龙岩市科技厅面上项目(2021LY-F7062); 福建中医药大学校管课题(XB2022159)
2026-05-22
2026-05-22