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目的 探讨小檗碱(BBR)对大鼠脊髓损伤后的保护作用及其可能机制。方法 将108只雌性SD大鼠随机分为假手术组(Sham)、脊髓损伤溶剂组(SCI+Veh)和脊髓损伤BBR组(SCI+BBR)。采用Allen打击法制备脊髓损伤(SCI)模型。脊髓损伤3 d后，采用Western印迹、实时荧光定量聚合酶链反应(qPCR)及免疫荧光染色技术，定量分析BBR(50 mg/kg)对损伤区自噬相关蛋白、炎症因子mRNA、细胞凋亡和神经元细胞密度变化的影响，并通过血脑屏障(BBB)评分对后肢运动功能的恢复情况进行综合评估。结果 与Sham组相比，SCI+Veh组和SCI+BBR组自噬相关基因微管相关蛋白轻链(LC)3Ⅱ,自噬相关蛋白(Beclin)-1蛋白表达量及促炎因子肿瘤坏死因子(TNF)-α,CD86,白细胞介素(IL)-1β,诱导型一氧化氮合酶(iNOS)mRNA表达均显著升高(P<0.05);损伤区及周围组织出现大量原位末端转移酶标记法(TUNEL)阳性细胞且神经元细胞密度显著减少(P<0.05)。与SCI+Veh组相比，BBR显著性促进了损伤区自噬相关蛋白表达(P<0.05);显著抑制促炎因子mRNA表达(P<0.05),明显减少了细胞凋亡及神经元数量丢失(P<0.05)并显著促进了脊髓损伤21～42 d后肢运动功能恢复(P<0.05)。结论 SCI后，BBR通过增强损伤区的自噬活性抑制了炎症反应，进而减少细胞凋亡和神经元细胞丢失，促进了大鼠后肢运动功能恢复。
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基本信息:
中图分类号:R285.5
引用信息:
[1]黄海梅,余丹,刘春苗,等.小檗碱通过促进自噬对大鼠脊髓损伤后的保护作用[J].中国老年学杂志,2026,46(06):1090-1094.
基金信息:
海南省卫生健康行业科研项目(20A200061)
2026-03-25
2026-03-25