| 29 | 0 | 7 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探究万古霉素对脂多糖(LPS)刺激肺癌细胞炎症反应、NOD样受体蛋白(NLRP)3/半胱氨酸蛋白酶(Caspase)-1信号的影响。方法 收集人肺癌A549细胞常规培养后将其分为对照组(经LPS诱导的A549细胞)、10、20、40μg/ml组(万古霉素10μg/ml+经LPS诱导的A549细胞)。采用酶联免疫吸附试验(ELISA)检测各组细胞中白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α、IL-6水平，采用Hoechst33342染色检测细胞凋亡形态，采用Transwell试验检测细胞侵袭；采用划痕试验检测细胞迁移；Western检测细胞中NLRP3、Caspase-1蛋白表达。结果 与对照组比较，10、20、40μg/ml组IL-1β、TNF-α、IL-6、细胞侵袭数量、NLRP3、Caspase-1蛋白、划痕愈合率明显降低，呈剂量依赖性(P<0.05);细胞凋亡形态显示，对照组可见明显细胞核固缩、团块现象；与对照组相比，10、20、40μg/ml组核固缩细胞数升高，其中40μg/ml组核固缩细胞数升高显著。结论 万古霉素对降低LPS诱导肺癌细胞引发的炎症反应和调节NLRP3/Caspase-1信号通路具有一定积极效用。
Abstract：Objective To investigate the effects of vancomycin on the inflammatory response and NOD-like receptor protein(NLRP)3/Caspase-1 signaling in lung cancer cells stimulated by lipopolysaccharide(LPS).Methods The human lung cancer A549 cells were cultured routinely and then divided into the control group(A549 cells induced by LPS),and the 10,20 and 40 μg/ml groups(10 μg/ml vancomycin+A549 cells induced by LPS).The enzyme-linked immunosorbent assay(ELISA) was used to detect levels of interleukin(IL)-1β,tumor necrosis factor(TNF)-α and IL-6 in the cells of each group.The apoptosis morphology of cells was detected by Hoechst33342 staining and the cell invasion was examined by Transwell assay.The scratch assay was used to detect cell migration, and Western blotting was employed to examine the protein expressions of NLRP3 and Caspase-1 in the cells.Results Compared with the control group, the levels of IL-1β,TNF-α,IL-6,the number of cell invasion, NLRP3,Caspase-1 protein and the scratch healing rate in the 10,20,and 40 μg/ml groups were significantly decreased, showing a dose-dependent pattern(P<0.05).The morphology of cell apoptosis showed that in the control group, obvious nuclear shrinkage and clumping phenomena were observed.Compared with the control group, the number of cells with nuclear pyknosis was increased in the 10,20,and 40 μg/ml groups, and the number of cells with nuclear pyknosis was significantly increased in the 40 μg/ml group.Conclusions Vancomycin has a positive effect on reducing LPS-induced inflammation in lung cancer cells and regulating NLRP3/Caspase-1 signaling pathway.
1 Tamanna MT,Egbune C.Traditional treatment approaches and role of immunotherapy in lung malignancy and mesothelioma[J].Cancer Treat Res,2023;185(1):79-89.
2 Qu Z,Liu J,Zhu L,et al.Clinical features and treatment modalities of rare choroid metastasis from lung malignancy[J].Chin Med J(Engl),2022;135(13):1628-30.
3 Zong F,Gan C,Wang Y,et al.Exposure to aerosolized staphylococcal enterotoxin B potentiated by lipopolysaccharide modifies lung transcriptomes and results in lung injury in the mouse model[J].J Appl Toxicol,2022;42(7):1205-17.
4 Wang J,Li G,Lin S,et al.Circ-BICC1 knockdown alleviates lipopolysaccharide(LPS)-Induced WI-38 cell injury through miR-338-3p/MYD88 axis[J].Biochem Genet,2023;61(1):170-86.
5 Chen M,Hu C,Yang L,et al.Saikosaponin-D induces the pyroptosis of lung cancer by increasing ROS and activating the NF-κB/NLRP3/caspase-1/GSDMD pathway[J].J Biochem Mol Toxicol,2023;37(8):e23444.
6 Yoshida Y,Fukuda T,Fukuoka K,et al.Time-dependent differences in vancomycin sensitivity of macrophages underlie vancomycin-induced acute kidney injury[J].J Pharmacol Exp Ther,2024;388(1):218-27.
7 刘云宁，汤建华，姜爱雯，等.万古霉素结合头孢曲松钠治疗肝癌手术并发复杂性腹腔感染的效果[J].热带医学杂志，2023;23(9):1266-9,1298.
8 王华.利奈唑胺联合万古霉素对重症肺炎患者肺功能及血清IL-1β、TGF-β、TNF-α水平的影响[J].基层医学论坛，2021;25(20):2856-8.
9 Xu Q,Zhang S,Quan J,et al.The evaluation of fecal microbiota transplantation vs vancomycin in a Clostridioides difficile infection model[J].Appl Microbiol Biotechnol,2022;106(19-20):6689-700.
10 Hees T,Abdelatif Y,Karpinski K,et al.Soaking ACL grafts in vancomycin solution(1 mg/ml) reduces the infection rate without increasing the risk for re-rupture and arthrofibrosis[J].Arch Orthop Trauma Surg,2022;142(6):1141-6.
11 Wang X,Cai H,Chen Z,et al.Baicalein alleviates pyroptosis and inflammation in hyperlipidemic pancreatitis by inhibiting NLRP3/Caspase-1 pathway through the miR-192-5p/TXNIP axis[J].Int Immunopharmacol,2021;101(Pt B):108315.
12 Lv YX,Pan HR,Song XY,et al.Hedyotis diffusa plus scutellaria barbata suppress the growth of non-small-cell lung cancer via NLRP3/NF-κB/MAPK signaling pathways[J].Evid Based Complement Alternat Med,2021;14(1):6666499.
13 梁笑天.万古霉素对慢性心力衰竭伴肺部感染患者血清炎症因子及脑钠肽水平的影响[J].现代诊断与治疗，2021;32(16):2556-7.
14 Jessica LB,Denis P,Sandra S,et al.Toward model-based informed precision dosing of vancomycin in hematologic cancer patients:a first step[J].Clin Pharmacokinet,2024;63(2):183-96.
15 Wang B,Shen XY,Pan LY,et al.The HDAC2-MTA3 interaction induces nonsmall cell lung cancer cell migration and invasion by targeting c-Myc and cyclin D1[J].Mol Carcinog,2023;62(11):1630-44.
16 杨世界.替考拉宁与万古霉素治疗肺癌患者耐甲氧西林金黄色葡萄球菌肺炎的效果观察[J].临床医药文献电子杂志，2020;7(10):171-3.
17 Shifko CR,Jenniches D,Holmberg K,et al.Antibiotic prophylaxis for grade 3 open fractures:a retrospective comparison of ceftriaxone plus vancomycin versus cefazolin plus gentamicin[J].Surg Infect(Larchmt),2024;25(1):39-45.
18 Wu W,Liu M,Geng JJ,et al.Teicoplanin combined with conventional vancomycin therapy for the treatment of pulmonary methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis infections[J].World J Clin Cases,2021;9(34):10549-56.
19 Yan Y,Yang G,Li Y,et al.Factorial design and post-antibiotic sub-MIC effects of linezolid combined with fosfomycin against vancomycin-resistant enterococci[J].Ann Transl Med,2022;10(3):148.
基本信息:
中图分类号:R734.2
引用信息:
[1]赵莉莉,王娜,贾佩佩,等.万古霉素对LPS刺激肺癌细胞炎症反应、NLRP3/Caspase-1信号的影响[J].中国老年学杂志,2026,46(06):1071-1075.
基金信息:
国家自然科学基金资助项目(82004314); 秦皇岛市科学技术局科研基金项目(202101A041)