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目的 研究瑞马唑仑通过调节Wnt/β-Catenin信号通路对喉鳞状细胞癌(LSCC)细胞恶性生物学行为的影响。方法 体外培养人喉鳞状细胞癌细胞AMC-HN-8,先用细胞计数(CCK-8)法检测瑞马唑仑对AMC-HN-8细胞的增殖能力影响，筛选合适的瑞马唑仑浓度，然后将AMC-HN-8细胞分为对照组、低浓度瑞马唑仑组(0.50μg/ml)、高浓度瑞马唑仑组(1.00μg/ml)、高浓度瑞马唑仑(1.00μg/ml)+Wnt/β-Catenin激活剂组(4 mmol/L Wnt/β-Catenin激活剂)。分组处理后，CCK-8法检测各组细胞活性；流式细胞仪检测细胞凋亡；Transwell小室检测细胞侵袭能力；创口愈合实验检测细胞迁移能力；Western印迹检测Wnt3a、β-Catenin、增殖细胞核抗原(PCNA)、B细胞淋巴瘤(Bcl)2蛋白表达。结果 与对照组比较，低、高浓度瑞马唑仑组细胞存活率降低、侵袭细胞数目减少、创口愈合率降低、Wnt3a、β-Catenin、PCNA、Bcl2、N-cadherin蛋白表达降低，细胞凋亡率、Cleaved-Caspase3、E-cadherin蛋白表达上升，差异有统计学意义(P<0.05);与高浓度瑞马唑仑组比较，高浓度瑞马唑仑+Wnt/β-Catenin激活剂组细胞存活率增加、细胞侵袭数目增多、创口愈合率升高、Wnt3a、β-Catenin、PCNA、Bcl2、N-cadherin蛋白表达升高，细胞凋亡率、Cleaved-Caspase3、E-cadherin蛋白表达降低，差异有统计学意义(P<0.05)。结论 瑞马唑仑可能通过对Wnt/β-Catenin信号通路的抑制作用，诱导LSCC细胞凋亡，并对细胞的增殖、迁移及侵袭能力产生抑制效应。
Abstract：1 Cavaliere M,Bisogno A,Scarpa A,et al.Biomarkers of laryngeal squamous cell carcinoma:a review[J].Ann Diagn Pathol,2021;54(1):151787-816.
2 Wang Z,Sun A,Yan A,et al.Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation[J].Mol Cancer,2022;21(1):92-111.
3 Lee B,Kim MH,Kong HJ,et al.Effects of Remimazolam vs.Sevoflurane anesthesia on intraoperative hemodynamics in patients with gastric cancer undergoing robotic gastrectomy:a propensity score-matched analysis[J].J Clin Med,2022;11(9):2643-54.
4 Liu Y,Wang S,Yang W.Inhibiting the proliferation of colorectal cancer cells by reducing TSPO/VDAC expression[J].Iran J Public Health,2023;52(7):1378-89.
5 Xu W,Hu J,Liu W,et al.Remimazolan inhibits glioma cell growth and induces apoptosis through down-regulation of NF-κB pathway[J].IUBMB Life,2021;73(2):341-8.
6 曲强，孙英杰，吴腾飞，等.五味子乙素抑制膀胱癌细胞增殖和侵袭转移及对Wnt/β-catenin信号通路的调节作用[J].解剖科学进展，2023;29(3):257-9,263.
7 Meng Q,Li L,Wang L.High CBX8 expression leads to poor prognosis in laryngeal squamous cell carcinoma by inducing EMT by activating the Wnt/β-Catenin signaling pathway[J].Front Oncol,2022;12(1):881262-172.
8 Liang J,Sun L,Li Y,et al.Wnt signaling modulator DKK4 inhibits colorectal cancer metastasis through an AKT/Wnt/β-catenin negative feedback pathway[J].J Biol Chem,2022;298(11):102545.
9 Mastronikolis N,Ragos V,Fotiades P,et al.mdm2 oncogene in laryngeal squamous cell carcinoma[J].J BUON,2020;25(2):594-6.
10 Kitamura N,Sento S,Yoshizawa Y,et al.Current trends and future prospects of molecular targeted therapy in head and neck squamous cell carcinoma[J].Int J Mol Sci,2020;22(1):240-52.
11 St?hr T,Colin PJ,Ossig J,et al.Pharmacokinetic properties of remimazolam in subjects with hepatic or renal impairment[J].Br J Anaesth,2021;127(3):415-23.
12 Xiao Y,Wei R,Chen L,et al.Efficacy and safety of remimazolam for procedural sedation during ultrasound-guided transversus abdominis plane block and rectus sheath block in patients undergoing abdominal tumor surgery:a single-center randomized controlled trial[J].BMC Anesthesiol,2022;22(1):381-90.
13 陈威弛，吴腾飞，刘永叶.桦木酸调控Wnt/β-catenin信号通路抑制肝癌细胞增殖、侵袭、转移和上皮间质转化[J].解剖科学进展，2022;28(6):719-21,726.
14 Bian J,Dannappel M,Wan C,et al.Transcriptional regulation of Wnt/β-Catenin pathway in colorectal cancer[J].Cells,2020;9(9):2125-53.
15 Wan L,Gu D,Li P.LncRNA SNHG16 promotes proliferation and migration in laryngeal squamous cell carcinoma via the miR-140-5p/NFAT5/Wnt/β-catenin pathway axis[J].Pathol Res Pract,2022;229(1):153727-42.
基本信息:
DOI：
中图分类号:R739.65
引用信息:
[1]蔡玉红,李辉,毛远兴等.瑞马唑仑调节Wnt/β-Catenin信号通路对喉鳞状细胞癌细胞恶性生物学行为的影响[J].中国老年学杂志,2025,45(13):3200-3204.
基金信息:
上海市崇明区第四轮医学重点学科(扶持学科)建设计划项目(CM(2023-2025)-0308-05)