| 28 | 0 | 4 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探讨甲基化CpG结合蛋白(MeCP)2对胶质瘤U87细胞的生物学影响。方法 应用siRNA干扰U87细胞MeCP2表达；采用实时荧光定量聚合酶链反应(qRT-PCR)法检测胶质瘤U87细胞中MeCP2含量；CCK-8法和集落形成法分析MeCP2对U87细胞增殖活力的影响；划痕实验和Transwell实验分别检测MeCP2对细胞迁移和侵袭能力的影响。结果 siRNA明显降低胶质瘤细胞U87中MeCP2表达(P<0.05);转染MeCP2 siRNA的U87细胞活力及增殖能力显著下降(P<0.05);MeCP2表达降低后U87细胞迁移和侵袭能力明显下降(P<0.05)。结论 MeCP2高表达能促进胶质瘤U87细胞增殖、迁移和侵袭。
Abstract：1 Thakur A,Faujdar C,Sharma R,et al.Glioblastoma:current status,emerging targets,and recent advances[J].J Med Chem,2022;65(13):8596-685.
2 Louis DN,Perry A,Wesseling P,et al.The 2021 WHO classification of tumors of the central nervous system:a summary[J].Neuro Oncol,2021;23(8):1231-51.
3 Stupp R,Mason WP,van den Bent MJ,et al.Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma[J].N Engl J Med,2005;352(10):987-96.
4 Stupp R,Hegi ME,Mason WP,et al.Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase Ⅲ study:5-year analysis of the EORTC-NCIC trial[J].Lancet Oncol,2009;10(5):459-66.
5 Kannan S,Murugan AK,Balasubramanian S,et al.Gliomas:genetic alterations,mechanisms of metastasis,recurrence,drug resistance,and recent trends in molecular therapeutic options[J].Biochem Pharmacol,2022;201:115090.
6 Louis DN,Perry A,Reifenberger G,et al.The 2016 World Health Organization classification of tumors of the central nervous system:a summary[J].Acta Neuropathol,2016;131(6):803-20.
7 Stupp R,Mason WP,van den Bent MJ,et al.Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma[J].N Engl J Med,2005;352(10):987-96.
8 Stupp R,Hegi ME,Mason WP,et al.Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase Ⅲ study:5-year analysis of the EORTC-NCIC trial[J].Lancet Oncol,2009;10(5):459-66.
9 Yao W,Lin S,Su J,et al.Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents[J].Transl Psychiatry,2021;11(1):140.
10 Feldman D,Banerjee A,Sur M.Developmental dynamics of rett syndrome[J].Neural Plast,2016;2016:6154080.
11 Neupane M,Clark AP,Landini S,et al.MECP2 is a frequently amplified oncogene with a novel epigenetic mechanism that mimics the role of activated RAS in malignancy[J].Cancer Discov,2016;6(1):45-58.
12 Parry L,Clarke AR.The roles of the methyl-CpG binding proteins in cancer[J].Genes Cancer,2011;2(6):618-30.
13 Jiang W,Liang YL,Liu Y,et al.MeCP2 inhibits proliferation and migration of breast cancer via suppression of epithelial-mesenchymal transition[J].J Cell Mol Med,2020;24(14):7959-67.
14 Sharma K,Singh J,Pillai PP.MeCP2 differentially regulate the myelin MBP and PLP protein expression in oligodendrocytes and C6 Glioma[J].J Mol Neurosci,2018;65(3):343-50.
15 Sharma K,Singh J,Frost EE,et al.MeCP2 overexpression inhibits proliferation,migration and invasion of C6 glioma by modulating ERK signaling and gene expression[J].Neurosci Lett,2018;674:42-8.
16 Bian E,Chen X,Xu Y,et al.A central role for MeCP2 in the epigenetic repression of miR-200c during epithelial-to-mesenchymal transition of glioma[J].J Exp Clin Cancer Res,2019;38(1):366.
基本信息:
DOI：
中图分类号:R739.41
引用信息:
[1]黄冠又,张欣,苏恒等.MeCP2对胶质瘤U87细胞增殖和侵袭的影响[J].中国老年学杂志,2025,45(13):3249-3252.
基金信息:
贵州省第八批高层次创新型人才贵阳市培养对象项目(筑科合同-GCC-[2024]018); 贵州省卫健委科学技术基金项目(gzwkj2025-057); 贵阳市第二人民医院(金阳医院)博士研究启动计划(BS[2024]05)